A transfer-RNA-derived small RNA regulates ribosome biogenesis

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

An mRNA-Derived Noncoding RNA Targets and Regulates the Ribosome

The structural and functional repertoire of small non-protein-coding RNAs (ncRNAs) is central for establishing gene regulation networks in cells and organisms. Here, we show that an mRNA-derived 18-nucleotide-long ncRNA is capable of downregulating translation in Saccharomyces cerevisiae by targeting the ribosome. This 18-mer ncRNA binds to polysomes upon salt stress and is crucial for efficien...

Dicer-Dependent Biogenesis of Small RNAs Derived from 7SL RNA

It has been reported that decreased Dicer expression leads to Alu RNAs accumulation in human retinal pigmented epithelium cells, and Dicer may process the endogenous SINE/B1 RNAs (the rodent equivalent of the primate Alu RNAs) into small interfering RNAs (siRNAs). In this study, we aimed to address whether Dicer can process Alu RNAs and their common ancestor, 7SL RNA. Using Solexa sequencing te...

Small Non-coding Transfer RNA-Derived RNA Fragments (tRFs): Their Biogenesis, Function and Implication in Human Diseases

tRNA-derived RNA fragments (tRFs) are an emerging class of non-coding RNAs (ncRNAs). A growing number of reports have shown that tRFs are not random degradation products but are functional ncRNAs made of specific tRNA cleavage. They play regulatory roles in several biological contexts such as cancer, innate immunity, stress responses, and neurological disorders. In this review, we summarize the...

Ribosome Biogenesis: Ribosomal RNA Synthesis as a Package Deal

Ribosome biogenesis encompasses a complicated series of events involving hundreds of transiently interacting components. Insight into a mechanism for coordinating some of these events may come from characterization of a functional processing complex.